Imaging Intracellular Signaling Using Two-Photon Fluorescent Lifetime Imaging Microscopy
نویسندگان
چکیده
منابع مشابه
Imaging intracellular signaling using two-photon fluorescent lifetime imaging microscopy.
The recent development of Förster resonance energy transfer (FRET) sensors and FRET imaging techniques permits visualization of the dynamics of intracellular signaling events with high spatiotemporal resolution. In particular, fluorescence lifetime imaging in combination with two-photon laser-scanning microscopy (two-photon fluorescence lifetime imaging microscopy [2pFLIM]) is a powerful tool t...
متن کاملMonitoring Photosensitizer Uptake Using Two Photon Fluorescence Lifetime Imaging Microscopy
Photodynamic Therapy (PDT) provides an opportunity for treatment of various invasive tumors by the use of a cancer targeting photosensitizing agent and light of specific wavelengths. However, real-time monitoring of drug localization is desirable because the induction of the phototoxic effect relies on interplay between the dosage of localized drug and light. Fluorescence emission in PDT may be...
متن کاملVisible-wavelength two-photon excitation microscopy for fluorescent protein imaging.
The simultaneous observation of multiple fluorescent proteins (FPs) by optical microscopy is revealing mechanisms by which proteins and organelles control a variety of cellular functions. Here we show the use of visible-light based two-photon excitation for simultaneously imaging multiple FPs. We demonstrated that multiple fluorescent targets can be concurrently excited by the absorption of two...
متن کاملHigh Speed Two-Photon Lifetime Imaging
Nonlinear optical techniques and in particular two photon excited fluorescence imaging have emerged as powerful tools for deep tissue imaging with subcellular resolution, brain mapping and 3D printing. At the same time, fluorescent lifetime imaging probes internal biochemical interactions and external environment of a molecule useful for DNA sequencing, detection of tumour margins necessary for...
متن کاملInnovative Methodology Photon counting, censor corrections, and lifetime imaging for improved detection in two-photon microscopy
Jonathan D. Driscoll, Andy Y. Shih, Satish Iyengar, Jeffrey J. Field, G. Allen White, Jeffrey A. Squier, Gert Cauwenberghs, and David Kleinfeld Department of Physics, University of California at San Diego, La Jolla, California; Department of Statistics, University of Pittsburgh, Pittsburgh, Pennsylvania; Department of Physics, Colorado School of Mines, Golden, Colorado; and Department of Bioeng...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2012
ISSN: 1559-6095
DOI: 10.1101/pdb.top072090